產品概述
脂聯素又被稱作Acrp30,AdipoQ,apM1,GBP28, Acrp30是一種分子量為30KD的脂肪細胞補體相關蛋白，基因轉錄物apM1是在脂肪組織中表達最豐富的轉錄物， GBP28是脂肪細胞特異性分泌的28KD凝膠蛋白，在維持機體葡萄糖和脂質平衡上有著重要的作用，脂聯素結構包括一個膠原樣區域組成的N-末端和球狀區域組成的C-末端，此區域有一段與補體因子C1q非常類似的序列，此外脂聯素還具有增加抗動脈粥樣硬化和抗炎癥等生物學效應，在機體產生疾病時血漿中的脂聯素水平會降低。
產品原理
本實驗采用雙抗體夾心ELISA法。抗人Adiponectin單抗包被于酶標板上，標本和標準品中的Adiponectin會與單抗結合形成免疫復合物，游離的成分被洗去；加入生物素化的抗人Adiponectin抗體，生物素化抗人Adiponectin抗體與酶標板上結合的標準品或樣本中的Adiponectin結合而形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有Adiponectin，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，Adiponectin濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中Adiponectin濃度。
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