產品概述
TNF-α，也稱為 TNFSFIA，是腫瘤壞死因子家族的成員，在炎癥反應與細胞凋亡中發揮重要作用。初始合成時是26kD分子量的233個氨基酸組成II型跨膜蛋白質，包含30個氨基酸組成的胞內區， 26 個氨基酸組成的跨膜區，以及177個氨基酸組成的胞外區。TNF-α胞內通過非共價的連接形成跨膜的同源二聚體。通過 TNF-α 轉換酶（TACE）的作用，從跨膜區C端釋放出一個由157個氨基酸組成的可溶 TNF-α（s TNF-α 。目前，已知表達TNF-α的人類細胞有：B細胞，結腸柱狀上皮細胞，NK細胞， CD3+CD56+肝自然T細胞，巨噬細胞，單核細胞，樹突狀細胞，CD4+ CD8+ T細胞，肥大細胞，嗜中性粒細胞，角化細胞，血漿細胞以及脂肪細胞等。
產品原理
本實驗采用雙抗體夾心ELISA法。抗人TNF-α單抗包被于酶標板上，標本和標準品中的TNF-α會與單抗結合形成免疫復合物，游離的成分被洗去；加入生物素化的抗人TNF-α抗體，生物素化抗人TNF-α抗體與酶標板上結合的標準品或樣本中的TNF-α結合而形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有TNF-α，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，TNF-α濃度與OD450值之間呈正相關，可通過繪制標準曲線求出標本中TNF-α濃度。
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